• 1.成都軍區(qū)總醫(yī)院全軍普外中心（成都610083）;;
  • 2.第一軍醫(yī)大學(xué)珠江醫(yī)院肝膽外科（廣州510282）;

目的  探討卵圓細(xì)胞在進(jìn)行性肝損傷過(guò)程中的分布及遷移規(guī)律。
方法  清潔型SD 大鼠60只隨機(jī)分為對(duì)照組（20只）和實(shí)驗(yàn)組（40只），兩組均于肝癌造模后的不同時(shí)相切取肝組織標(biāo)本進(jìn)行形態(tài)學(xué)觀察、常規(guī)病理和原癌基因編碼蛋白（C-kit）免疫組化檢測(cè)。
結(jié)果  對(duì)照組大鼠肝臟表面光滑，組織學(xué)形態(tài)正常，偶見C-kit陽(yáng)性細(xì)胞。實(shí)驗(yàn)組于肝癌造模后的第2周，首先于匯管區(qū)發(fā)現(xiàn)卵圓細(xì)胞沿膽管上皮依次排列增生，這些卵圓細(xì)胞呈C-kit陽(yáng)性表達(dá)。隨著肝損傷進(jìn)行性加重，卵圓細(xì)胞以匯管區(qū)為中心向肝小葉穿插、遷移。肝癌形成期，以混合型癌多見，癌結(jié)節(jié)內(nèi)外均見有卵圓細(xì)胞，此期C-kit陽(yáng)性細(xì)胞仍集中于匯管區(qū)。
結(jié)論  卵圓細(xì)胞為肝組織內(nèi)對(duì)損傷反應(yīng)最敏感的一種細(xì)胞； 肝卵圓細(xì)胞的無(wú)序遷移參與假小葉形成； 卵圓細(xì)胞與肝癌的發(fā)生密切相關(guān)。

引用本文： 龔加慶,方馳華,李雅. 肝卵圓細(xì)胞在進(jìn)行性肝損傷過(guò)程中分布及遷移的實(shí)驗(yàn)研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2004, 11(5): 389-392. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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  2. 2. Baker DG, Taylor HW, Lee SP, et al. Hepatic toxicity and recovery of Fischer 344 rats following exposure to 2aminoanthracene by intraperitoneal injection [Ｊ]. Toxicol Pathol, 2001; 29(3)∶328.
  3. 3. Petersen BE. Hepatic “stem” cells: coming full circle [Ｊ]. Blood Cells Mol Dis， 2001; 27(3)∶590.
  4. 4. Baumann U, Crosby HA, Ramani P, et al. Expression of the stem cell factor receptor ckit in normal and diseased pediatric liver: identification of a human hepatic progenitor cell? [Ｊ]. Hepatology, 1999; 30(1)∶112.
  5. 5. 龔加慶，方馳華.大鼠卵圓細(xì)胞與原發(fā)性肝細(xì)胞癌發(fā)生的關(guān)系 [Ｊ]. 世界華人消化雜志， 2002； 10（10）∶1133.
  6. 6. Hellerbrand C, Jobin C, Licato LL, et al. Cytokines induce NFkappaB in activated but not in quiescent rat hepatic stellate cells [Ｊ]. Am J Physiol, 1998; 275(2 Pt 1)∶G269.
  7. 7. McCrudden R, Iredale JP. Liver fibrosis, the hepatic stellate cell and tissue inhibitors of metalloproteinases [Ｊ]. Histol Histopathol, 2000; 15(4)∶1159.
  8. 8. Gelderblom WC, Marasas WF, LebepeMazur S, et al. Interaction of fumonisin B(1) and aflatoxin B(1) in a shortterm carcinogenesis model in rat liver [Ｊ]. Toxicology, 2002; 171(2-3)∶161.
  9. 9. Ahn B, Han BS, Kim DJ, et al. Immunohistochemical localization of inducible nitric oxide synthase and 3nitrotyrosine in rat liver tumors induced by Nnitrosodiethylamine [Ｊ]. Carcinogenesis, 1999; 20(7)∶1337.