• 1. 四川省瀘州醫(yī)學(xué)院附屬醫(yī)院胃腸外科(瀘州646000)2. 中山醫(yī)科大學(xué)附一院;

應(yīng)用流式細胞術(shù)和細胞免疫熒光染色技術(shù)，對46例大腸腫瘤細胞ras p21表達和DNA含量進行了定量分析。結(jié)果：大腸癌的ras p21表達陽性率為65.7%，DNA異倍體率為74.3%，大腸癌細胞ras p21表達量明顯高于腺瘤和正常粘膜；DNA倍體和細胞增殖指數(shù)值與ras p21表達量密切相關(guān)。本實驗結(jié)果提示：測定大腸癌的ras p21表達和DNA含量將有助于其預(yù)后判斷。

引用本文： 徐亮,盧光宇. 大腸癌ras p21表達和DNA含量定量分析. 中國普外基礎(chǔ)與臨床雜志, 1998, 5(1): 4-6. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Hedley DM, Yonemura Y, Suzuki H, et al. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem, 1983; 31∶33.
2. Morkve O. Flow cytometric measurement of p53 protein expression and DNA content in paraffin embedded tissue from brochial carcinomas. Cytometry, 1991; 12∶438.
3. Barbacid M, Saito H, Clement JM, et al. Ras genes. Ann Rev Biochem,1987; 56∶779.
4. Bos JL. Ras oncogene in human cancer. Cancer Res, 1989; 49∶4682.
5. Scott N，Quirke P. Molecular biology of colorectal neoplasia. Gut, 1993; 34(3)∶289.
6. Miyahara M, Saito T, Kaketani K, et al. Clinical significance of ras p21 overexpression for patients with an advanced colorectal cancer. Dis Colon Rectum, 1991; 34∶1097.
7. Jansson DS. An immunohistochemical analysis of ras oncogene expression in epithelial neoplasms of the colon. Cancer, 1990; 65∶1329.
8. Wang Q. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinoma of the rectum and rectosigmoid. Dis Colon Rectum, 1992; 25∶670.
9. Merkel DE， Mcguire WL. Ploidy proliferative activity and prognosis， DNA flow cytometry of solid tumors. Cancer, 1990; 65∶1194.
10. Barbara F, Chacho MS, Rosemen DL, et al. Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol,1987; 87∶313.
11. Quirke P， Fozard JBJ, Dixon MF, et al. DNA aneuploidy in human colon carcinomas. Br J Cancer, 1986; 53∶477.
12. Sun XF. Ras p21 expression in relation to DNA ploidy, Sphase frac-tion and prognosis in colorectal adenocarcinomas.Cytometry, 1991; Suppl 5∶59.
13. Michelasis F, Erroi F, Roncella M, et al. Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma. Dis Colon Rectum, 1989; 32∶665.
  1. 1. Hedley DM, Yonemura Y, Suzuki H, et al. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem, 1983; 31∶33.
  2. 2. Morkve O. Flow cytometric measurement of p53 protein expression and DNA content in paraffin embedded tissue from brochial carcinomas. Cytometry, 1991; 12∶438.
  3. 3. Barbacid M, Saito H, Clement JM, et al. Ras genes. Ann Rev Biochem,1987; 56∶779.
  4. 4. Bos JL. Ras oncogene in human cancer. Cancer Res, 1989; 49∶4682.
  5. 5. Scott N，Quirke P. Molecular biology of colorectal neoplasia. Gut, 1993; 34(3)∶289.
  6. 6. Miyahara M, Saito T, Kaketani K, et al. Clinical significance of ras p21 overexpression for patients with an advanced colorectal cancer. Dis Colon Rectum, 1991; 34∶1097.
  7. 7. Jansson DS. An immunohistochemical analysis of ras oncogene expression in epithelial neoplasms of the colon. Cancer, 1990; 65∶1329.
  8. 8. Wang Q. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinoma of the rectum and rectosigmoid. Dis Colon Rectum, 1992; 25∶670.
  9. 9. Merkel DE， Mcguire WL. Ploidy proliferative activity and prognosis， DNA flow cytometry of solid tumors. Cancer, 1990; 65∶1194.
  10. 10. Barbara F, Chacho MS, Rosemen DL, et al. Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol,1987; 87∶313.
  11. 11. Quirke P， Fozard JBJ, Dixon MF, et al. DNA aneuploidy in human colon carcinomas. Br J Cancer, 1986; 53∶477.
  12. 12. Sun XF. Ras p21 expression in relation to DNA ploidy, Sphase frac-tion and prognosis in colorectal adenocarcinomas.Cytometry, 1991; Suppl 5∶59.
  13. 13. Michelasis F, Erroi F, Roncella M, et al. Ras oncogene and the acquisition of metastasizing properties by rectal adenocarcinoma. Dis Colon Rectum, 1989; 32∶665.