• 1 四川省醫(yī)學(xué)科學(xué)院　四川省人民醫(yī)院婦產(chǎn)科（成都，610072）;2 四川大學(xué)華西醫(yī)院眼科;

【摘要】　目的　 研究ΔNP63/TAP63在上皮性卵巢腫瘤組織中的表達(dá)及其與臨床病理特征的關(guān)系。　方法　 運(yùn)用熒光定量聚合酶鏈反應(yīng)方法檢測2002年-2004年54例卵巢上皮性腫瘤中ΔNP63/TAP63的基因水平。　結(jié)果　 33例卵巢上皮細(xì)胞癌組織中ΔNP63的表達(dá)高于21例良性上皮性腫瘤中組織。卵巢上皮細(xì)胞癌中的表達(dá)強(qiáng)度與腫瘤組織病理學(xué)分期相關(guān)（P lt;0.05），良性腫瘤的表達(dá)低于惡性腫瘤（P lt;0.05）。ΔNP63的表達(dá)高于TAP63（P lt;0.05）；各組間TAP63的表達(dá)差異無統(tǒng)計學(xué)意義（P gt;0.05）。　結(jié)論　 ΔNP63在上皮性卵巢癌中高表達(dá)，可能成為上皮性卵巢癌診斷及預(yù)后的分子標(biāo)志物。
【Abstract】　Objective　 To explore the expression of ΔNP63 / TAP63 in human epithelial ovarian tumor tissues and its relationship with the clinicopathological features. 　Methods　 Fluorescent quantitative PCR method was used to detect 54 cases of ΔNP63 / TAP63 gene level in 54 patients with epithelial ovarian tumors diagnosed between 2002 and 2004.　Results　 ΔNP63 expression in the 33 cases of ovarian epithelial cell carcinoma was higher than that in the 21 cases of benign epithelial tumor tissue. The expression in ovarian epithelial cell carcinoma was concerned with the pathological staging of tumor (P lt;0.05); the expression in benign tumors was lower than that in the malignant tumors (P lt;0.05). In all cases, the expression of ΔNP63 was higher than that of TAP63 (P lt;0.05); the difference in the expression of TAP63 among the groups was not significant (P gt;0.05).　Conclusion　 ΔNP63 in epithelial ovarian cancer is highly expressed, which may become the molecular makers with diagnosis and prognosis of epithelial ovarian cancer diagnosis in the future.

引用本文： 王滟,楊業(yè)洲,謝蘭,楊年,郭波. ΔNP63/TAP63在卵巢上皮性腫瘤組織中的表達(dá)及其與臨床病理關(guān)系. 華西醫(yī)學(xué), 2011, 26(9): 1379-1381. doi: 復(fù)制

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12. 　Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63αand TAP63αregulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357.
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  2. 2. 　Kocken M, Baalbergen A, Snijders PJ, et al. High-risk human papillomavirus seems not involved in DES-related and of limited importance in nonDES related clear-cell carcinoma of the cervix. [J]. Gynecol Oncol, 2011, 122(2): 297-302.
  3. 3. 　王曉惠, 彭芝蘭, 雷軍強(qiáng), 等. 卡鉑誘導(dǎo)卵巢癌細(xì)胞株OVCA_3凋亡中p63及p53基因mRNA的變化[J]. 蘭州大學(xué)學(xué)報(醫(yī)學(xué)版), 2010, 36(4): 8-10.
  4. 4. 　Osada M, Ohba M, Kawabara C, et al. Cloning and functional analysis of human P51,　which structurally and functional resembles P53[J]. Nat Med, 1998, 7(4): 839-843.
  5. 5. 　Levrero M, Laurenzi VD, Costanzo A, et al. Structure,function and regulation of P63 and P73[J]. Cell Death Differ [J], 1999, 6(12): 1154-1161.
  6. 6. 　Irwin MS, Kaelin WG. Role of the newer P53 family proteins in malignancy[J]. Apoptosis, 2001, 6(10): 17-19.
  7. 7. 　Parsa R, Yang A, McKeon F, et al. Association of P63 with proliferative potential in n ormal and neoplastic human keratinocytes[J]. J Invest Dermatol, 1999, 113(10): 1099-1105.
  8. 8. 　Glickman JN, Yang A, Shahsafaei A, et al. Expression of P53-related protein P63 in the gastrointestinal tract and in esophageal metaplastic disorders [J]. Hum Pathol, 2001, 32(11): 1157-1165.
  9. 9. 　Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63α and TAP63α regulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357.
  10. 10. 　司少艷, 張建中. P63基因研究進(jìn)展[J]. 世界華人消化雜志, 2003, 11(5): 606-609.
  11. 11. 　Elsa R, Flores, Kenneth Y, et al. P63 and P73 are required for P53 dependent apoptosis in response to DNA damage[J]. Nature, 2002, 416(4): 560-564.
  12. 12. 　Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63αand TAP63αregulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357.