• 四川大學華西醫(yī)院急診科,四川成都 610041;

目的:探討貫葉連翹提取物對百草枯誘導的大鼠肺纖維化模型的干預作用及機制。方法:20只Sprague-dawly大鼠隨機等分為4組:百草枯組,貫葉連翹提取物組,百草枯+貫葉連翹提取物組和對照組。百草枯液按80mg/kg一次性灌胃，貫葉連翹提取物按400mg/kg灌胃，連用3d,對照組僅用生理鹽水。于21d處死后取肺臟行HE染色鑒定,并用堿水解法測羥脯氨酸含量,同時以八木國夫熒光法測定肺組織脂質過氧化產(chǎn)物,鹽酸羥胺法測定組織總超氧化物歧化酶的酶活力。結果:百草枯染毒動物可致肺纖維化,羥脯氨酸和丙二醛含量明顯增高。百草枯+貫葉連翹提取物組與百草枯組比較,第21天時肺纖維化減輕,羥脯氨酸和丙二醛含量減少(P lt;0.05),與對照組相比總超氧化物歧化酶無明顯變化(P gt;0.05)。結論:貫葉連翹提取物對百草枯誘導的大鼠肺纖維化模型有抑制作用,其機制可能與抑制脂質過氧化有關。

引用本文： 何斌,杜曉冬,曹鈺. 貫葉連翹提取物對百草枯誘導肺纖維化保護作用的實驗研究. 華西醫(yī)學, 2009, 24(6): 1473-1475. doi: 復制

版權信息： ?四川大學華西醫(yī)院華西期刊社《華西醫(yī)學》版權所有，未經(jīng)授權不得轉載、改編

1. 魏薇,何慶.貫葉連翹提取物對百草枯所致的急性肺微血管內(nèi)皮細胞損傷的保護作用[J].四川大學學報(醫(yī)學版),2006,37(3):434-437.
2. 高燕,雍政,黃英,等.百草枯建立肺纖維化動物模型的實驗研究[J].空軍總醫(yī)院學報,2003,19(4):201-202.
3. ORITO K, SUZUKI Y, MATSUDA H, et al. Chymase is activated in the pulmonary inflammation and fibrosis induced by paraquat in hamsters[J]. Tohoku J Exp Med,2004,203(4):287-294.
4. ADACHI J, ISHII K, TOMITA M, et al. Consecutive administration of paraquat to rats induces enhanced cholesterol peroxidation and lung injury[J]. Arch Toxicol,2003,77(6):353-357.
5. CZINNER E, HAGYMA′SI K, BLA′ZOVICS A, et al. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation[J]. J Ethnopharmacol,2001,77:31-35.
6. CAKIR A, MAVI A, YILDIRIM A, et al. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L by activityguided fractionation[J]. J Ethnopharmacol,2003,87(1):73-83.
7. RUSSEL P B, JAMES D C． Oxidative stress in airways: Is there a role for extracelular superoxide dismutase?[J]Am J Respir Crit Care Med,2002,166(12):38-43.
  1. 1. 魏薇,何慶.貫葉連翹提取物對百草枯所致的急性肺微血管內(nèi)皮細胞損傷的保護作用[J].四川大學學報(醫(yī)學版),2006,37(3):434-437.
  2. 2. 高燕,雍政,黃英,等.百草枯建立肺纖維化動物模型的實驗研究[J].空軍總醫(yī)院學報,2003,19(4):201-202.
  3. 3. ORITO K, SUZUKI Y, MATSUDA H, et al. Chymase is activated in the pulmonary inflammation and fibrosis induced by paraquat in hamsters[J]. Tohoku J Exp Med,2004,203(4):287-294.
  4. 4. ADACHI J, ISHII K, TOMITA M, et al. Consecutive administration of paraquat to rats induces enhanced cholesterol peroxidation and lung injury[J]. Arch Toxicol,2003,77(6):353-357.
  5. 5. CZINNER E, HAGYMA′SI K, BLA′ZOVICS A, et al. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation[J]. J Ethnopharmacol,2001,77:31-35.
  6. 6. CAKIR A, MAVI A, YILDIRIM A, et al. Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L by activityguided fractionation[J]. J Ethnopharmacol,2003,87(1):73-83.
  7. 7. RUSSEL P B, JAMES D C． Oxidative stress in airways: Is there a role for extracelular superoxide dismutase?[J]Am J Respir Crit Care Med,2002,166(12):38-43.