摘要:目的:研究垂體瘤轉(zhuǎn)化基因在非小細(xì)胞肺癌組織、肺良性病變組織和正常支氣管黏膜上皮組織中的表達(dá), 初步探討其與非小細(xì)胞肺癌發(fā)生發(fā)展,侵襲轉(zhuǎn)移的關(guān)系。方法:(1)用SP免疫組化法檢測(cè)76例臨床石蠟組織標(biāo)本(44例非小細(xì)胞肺癌、20例肺良性病變組織和12例正常支氣管黏膜上皮組織)中的PTTG蛋白的表達(dá)。(2)用RTPCR法分析PTTG mRNA在不同性質(zhì)肺組織中的表達(dá)情況。〖HTH〗結(jié)果〖HTSS〗:(1)PTTG蛋白在不同性質(zhì)肺組織中的表達(dá)差別具有明顯差異;在TNM分期、淋巴結(jié)轉(zhuǎn)移組間差別有統(tǒng)計(jì)學(xué)意義。(2)PTTG mRNA在不同性質(zhì)肺組織中的表達(dá)差別具有明顯差異(P lt;0.001);在TNM分期、淋巴結(jié)轉(zhuǎn)移組間差別有統(tǒng)計(jì)學(xué)意義。結(jié)論：細(xì)胞肺癌的發(fā)生發(fā)展及轉(zhuǎn)移有關(guān)。
Abstract: Objective: To investigate the association between PTTG expression and biological behaviors of human nonsmall cell lung cancer (NSCLC).Methods:SP immunohistochemistry was used to detect the expression of PTTG in 76 cases of paraffinembedded specimens (including 44 surgical specimens from NSCLC patients, 20 pneumonic benign lesion and 19 normal bronchial epithelium).Realtime RTPCR was performed to detect PTTGm RNA expression in 44 cases of fresh carcinoma and corresponding adjacent normal mucosa specimens.Results: The expression levels of PTTG was significantly different between normal mucosa and adenoma tissues.There were statistical relationships between their expressions and TNM stage, lymphnode metastasis. Conclusion: PTTG may play an important role in carcinogenesis、development and metastasis of human nonsmall cell lung cancer.

引用本文： 王麗芳,郜紅利. 非小細(xì)胞肺癌中垂體瘤轉(zhuǎn)化基因的表達(dá)及臨床意義. 華西醫(yī)學(xué), 2009, 24(11): 2938-2940. doi: 復(fù)制

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