• 哈爾濱醫(yī)科大學(xué)附屬第二醫(yī)院普外七科（哈爾濱 150086）;

目的  研究腹腔應(yīng)用氟尿嘧啶（FU）對(duì)腫瘤動(dòng)物模型CO2氣腹操作后腫瘤細(xì)胞的生長(zhǎng)與轉(zhuǎn)移的作用。
方法  選取雄性清潔級(jí)H-22 小鼠50只，全部接種S-180細(xì)胞懸液，平均分成5組： 單純種植組、氣腹組、氣腹+生理鹽水組、氣腹+低濃度（5.0 g/L）FU組和氣腹+高濃度（10.0 g/L）FU組。11 d后全部處死，觀察腫瘤腹壁種植情況和瘤重（腹水重量差值），并通過(guò)免疫組化方法檢測(cè)增殖細(xì)胞核抗原（PCNA）和血管內(nèi)皮生長(zhǎng)因子（VEGF）的表達(dá)水平。
結(jié)果  除氣腹+低濃度FU組外，其余3組的瘤重均明顯高于氣腹+高濃度FU組，差異有統(tǒng)計(jì)學(xué)意義（P＜0.05或P＜0.01）。氣腹+高濃度FU組抑瘤率為64.5%，氣腹+低濃度FU組抑瘤率為43.3%。氣腹+生理鹽水組穿刺孔種植瘤結(jié)節(jié)直徑明顯大于氣腹+高濃度FU組和氣腹+低濃度FU組，差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）。各組小鼠腹水中腫瘤細(xì)胞和種植瘤結(jié)節(jié)中PCNA和VEGF表達(dá)的差異均有統(tǒng)計(jì)學(xué)意義（P＜0.05，P＜0.01）。
結(jié)論  腹腔內(nèi)應(yīng)用高濃度的FU對(duì)于S180細(xì)胞氣腹動(dòng)物模型的瘤重、穿刺孔種植瘤結(jié)節(jié)有抑制作用，其機(jī)理可能與下調(diào)PCNA和VEGF的表達(dá)有關(guān)。

引用本文： 孫鐵為,李強(qiáng),王鋒. 一次性CO2氣腹環(huán)境下氟尿嘧啶對(duì)大鼠腫瘤細(xì)胞生長(zhǎng)及轉(zhuǎn)移的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2009, 16(7): 545-549. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Volz J, Kster S, Schaeff B, et al. Laparoscopic surgery: the effects of insufflation gas on tumorinduced lethality in nude mice [Ｊ]. Am J Obstet Gynecol, 1998; 178(4): 793795.
2. 王廣義， 孟憲瑛， 顧建華， 等. CO2氣腹對(duì)腫瘤細(xì)胞種植與生長(zhǎng)的影響 [Ｊ]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2004; 11(2): 133135.
3. Cavina E, Goletti O, Molea N, et al. Trocar site tumor recurrences. May pneumoperitoneum be responsible? [Ｊ]. Surg Endosc, 1998; 12(11): 12941296.
4. Ridgway PF, Smith A, Ziprin P, et al. Pneumoperitoneum augmented tumor invasiveness is abolished by matrix metalloproteinase blockade [Ｊ]. Surg Endosc, 2002; 16(3): 533536.
5. 韋艾凌， 唐健. 癌痛消膠囊調(diào)節(jié)小鼠荷H22移植性肝癌細(xì)胞VEGF表達(dá)的實(shí)驗(yàn)研究 [Ｊ]. 廣西中醫(yī)藥, 2004; 27(4): 4749.
6. Texler ML, King G, Hewett PJ. Tumor cell movement during heating and humidification of insufflating CO2: an in vitro model [Ｊ]. Aust N Z J Surg, 1998; 68(10): 740742.
7. Wittich P, Steyerberg EW, Simons SH, et al. Intraperitoneal tumor growth is influenced by pressure of carbon dioxide pneumoperitoneum[Ｊ]. Surg Endosc, 2000; 14(9): 817819.
8. 王灝, 鄭民華, 張浩波, 等. CO2氣腹對(duì)人腫瘤細(xì)胞腹腔鏡戳口種植的影響 [Ｊ]. 外科理論與實(shí)踐, 1999; 4(3): 141143.
9. Kuntz C, Wunsch A, Bdeker C, et al. Effect of pressure and gas type on intraabdominal, subcutaneous, and blood pH in laparoscopy[Ｊ]. Surg Endosc, 2000; 14(4): 367371.
10. Lee SW, Whelan RL, Southall JC, et al. Abdominal wound tumor recurrence after open and laparoscopicassisted splenectomy in a murine model [Ｊ]. Dis Colon Rectum, 1998; 41(7): 824831.
11. Neuhaus SJ, Watson DI, Ellis T, et al. Influence of cytotoxic agents on intraperitoneal tumor implantation after laparoscopy[Ｊ]. Dis Colon Rectum, 1999; 42(1): 1015.
12. Krishna TS, Kong XP, Gary S, et al. Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA [Ｊ]. Cell, 1994; 79(7): 12331243.
13. Waga S, Hannon GJ, Beach D, et al. The p21 inhibitor of cyclindependent kinases controls DNA replication by interaction with PCNA [Ｊ]. Nature, 1994; 369(6481): 574578.
14. 孫曉敏, 董衛(wèi)國(guó), 余保平, 等. 檢測(cè)惡性腹水中VEGF、CD44v6和MMP2、MMP9的臨床意義 [Ｊ]. 癌癥, 2004; 23(1): 8589.
15. Mori A, Arii S, Furutani M, et al. Soluble Flt1 gene therapy for peritoneal metastases using HVJcationic liposomes [Ｊ]. Gene Ther, 2000; 7(12): 10271033.
  1. 1. Volz J, Kster S, Schaeff B, et al. Laparoscopic surgery: the effects of insufflation gas on tumorinduced lethality in nude mice [Ｊ]. Am J Obstet Gynecol, 1998; 178(4): 793795.
  2. 2. 王廣義， 孟憲瑛， 顧建華， 等. CO2氣腹對(duì)腫瘤細(xì)胞種植與生長(zhǎng)的影響 [Ｊ]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2004; 11(2): 133135.
  3. 3. Cavina E, Goletti O, Molea N, et al. Trocar site tumor recurrences. May pneumoperitoneum be responsible? [Ｊ]. Surg Endosc, 1998; 12(11): 12941296.
  4. 4. Ridgway PF, Smith A, Ziprin P, et al. Pneumoperitoneum augmented tumor invasiveness is abolished by matrix metalloproteinase blockade [Ｊ]. Surg Endosc, 2002; 16(3): 533536.
  5. 5. 韋艾凌， 唐健. 癌痛消膠囊調(diào)節(jié)小鼠荷H22移植性肝癌細(xì)胞VEGF表達(dá)的實(shí)驗(yàn)研究 [Ｊ]. 廣西中醫(yī)藥, 2004; 27(4): 4749.
  6. 6. Texler ML, King G, Hewett PJ. Tumor cell movement during heating and humidification of insufflating CO2: an in vitro model [Ｊ]. Aust N Z J Surg, 1998; 68(10): 740742.
  7. 7. Wittich P, Steyerberg EW, Simons SH, et al. Intraperitoneal tumor growth is influenced by pressure of carbon dioxide pneumoperitoneum[Ｊ]. Surg Endosc, 2000; 14(9): 817819.
  8. 8. 王灝, 鄭民華, 張浩波, 等. CO2氣腹對(duì)人腫瘤細(xì)胞腹腔鏡戳口種植的影響 [Ｊ]. 外科理論與實(shí)踐, 1999; 4(3): 141143.
  9. 9. Kuntz C, Wunsch A, Bdeker C, et al. Effect of pressure and gas type on intraabdominal, subcutaneous, and blood pH in laparoscopy[Ｊ]. Surg Endosc, 2000; 14(4): 367371.
  10. 10. Lee SW, Whelan RL, Southall JC, et al. Abdominal wound tumor recurrence after open and laparoscopicassisted splenectomy in a murine model [Ｊ]. Dis Colon Rectum, 1998; 41(7): 824831.
  11. 11. Neuhaus SJ, Watson DI, Ellis T, et al. Influence of cytotoxic agents on intraperitoneal tumor implantation after laparoscopy[Ｊ]. Dis Colon Rectum, 1999; 42(1): 1015.
  12. 12. Krishna TS, Kong XP, Gary S, et al. Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA [Ｊ]. Cell, 1994; 79(7): 12331243.
  13. 13. Waga S, Hannon GJ, Beach D, et al. The p21 inhibitor of cyclindependent kinases controls DNA replication by interaction with PCNA [Ｊ]. Nature, 1994; 369(6481): 574578.
  14. 14. 孫曉敏, 董衛(wèi)國(guó), 余保平, 等. 檢測(cè)惡性腹水中VEGF、CD44v6和MMP2、MMP9的臨床意義 [Ｊ]. 癌癥, 2004; 23(1): 8589.
  15. 15. Mori A, Arii S, Furutani M, et al. Soluble Flt1 gene therapy for peritoneal metastases using HVJcationic liposomes [Ｊ]. Gene Ther, 2000; 7(12): 10271033.