• 中國醫(yī)學科學院,中國協(xié)和醫(yī)科大學,北京協(xié)和醫(yī)院基本外科(北京100730);

目的  通過WST-8和膠原凝膠微滴腫瘤藥物敏感性檢測(CD-DST) 2種藥敏檢測方法的比較，對CD-DST法進行評價。方法  以胰腺癌細胞系SW1990、PCT-3和ASPC-1為對象，分別采用WST-8和CD-DST法測定其對5-氟尿嘧啶（5-FU）、健擇（GEM）和奧沙利鉑（OXA）3種藥物的敏感性。結果  在一定的活細胞數(shù)目范圍內（500~10 000個），CD-DST法所測得的細胞積分光密度與細胞數(shù)目成線性正相關（r=0.991 1, P＜0.05）； 3種腫瘤細胞的生長抑制率CD-DST法均高于WST-8法(P＜0.05)，2種方法測定的5-FU、GEM和OXA藥物敏感性結果一致。結論  CD-DST法可用于胰腺癌的體外藥敏檢測，具有一定的臨床應用價值。

引用本文： 周濤,胡亞,廖泉,趙玉沛. 2種體外腫瘤細胞藥敏檢測方法的比較研究. 中國普外基礎與臨床雜志, 2008, 15(11): 825-828. doi: 復制

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  3. 3. Kobayashi H, Higashiyama M, Minamigawa K, et al. Examination of in vitro chemosensitivity test using collagen gel droplet culture method with colorimetric endpoint quantification [J]. Jpn J Cancer Res, 2001; 92(2)∶203.
  4. 4. Alley MC, Scudiero DA, Monks A, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay [J]. Cancer Res, 1988; 48(3)∶589.
  5. 5. Yang J, Richards J, Bowman P, et al. Sustained growth and three-dimensional organization of primary mammary tumor epithelial cells embedded in collagen gels [J]. Proc Natl Acad Sci USA, 1979; 76(7)∶3401.
  6. 6. Richards J, Larson L, Yang J, et al. Method for culturing mammary epithelial cells in a rat tail collagen gel matrix [J]. Methods Cell Sci, 1983; 8(1)∶ 31.
  7. 7. Kawamura M, Gika M, Abiko T, et al. Clinical evaluation of chemosensitivity testing for patients with unresectable non-small cell lung cancer (NSCLC) using collagen gel droplet embedded culture drug sensitivity test (CD-DST) [J]. Cancer Chemother Pharmacol, 2007; 59(4)∶507.
  8. 8. Vaccarello L, Wang YL, Whiteside TL. Sustained outgrowth of autotumor-reactive T lymphocytes from human ovarian carcinomas in the presence of tumor necrosis factor alpha and interleukin 2 [J]. Hum Immunol, 1990; 28(2)∶216.
  9. 9. Sieuwerts AM, Klijn JG, Peters HA, et al. The MTT tetrazolium salt assay scrutinized: how to use this assay reliably to measure metabolic activity of cell cultures in vitro for the assessment of growth characteristics, IC50-values and cell survival [J]. Eur J Clin Chem Clin Biochem, 1995; 33(11)∶813.
  10. 10. Fricker SP, Buckley RG. Comparison of two colorimetric assays as cytotoxicity endpoints for an in vitro screen for antitumour agents [J]. Anticancer Res, 1996; 16(6B)∶3755.
  11. 11. Chen CF, Hwang JM, Wu CH, et al. Evaluation of a rapid tetrazolium-based colorimetric assay for selecting anticancer drugs [J]. Zhonghua Yi Xue Za Zhi (Taipei), 1990; 46(1)∶7.