• 1.南華大學(xué)第二附屬醫(yī)院普外科（湖南衡陽(yáng)421001）;;
  • 2.中南大學(xué)湘雅二醫(yī)院肝膽外科（長(zhǎng)沙410011）;

目的探討多次熱療后殘存的肝癌HepG2細(xì)胞增殖速度變化及其相關(guān)的可能原因。
方法HepG2細(xì)胞43 ℃加熱，每次80 min，2次/d，10個(gè)循環(huán)，分析細(xì)胞倍增時(shí)間，檢測(cè)細(xì)胞周期及bcl-2 mRNA及bax mRNA的表達(dá)。
結(jié)果熱處理后的HepG2細(xì)胞增殖加快，細(xì)胞周期中G2期和S期細(xì)胞的比率增高，bcl-2 mRNA的表達(dá)增強(qiáng)，bcl-2/bax比值增大。
結(jié)論熱處理后的HepG2細(xì)胞的增殖加快與其完成DNA復(fù)制而進(jìn)入分裂的細(xì)胞比率高、抗凋亡基因bcl-2 mRNA的強(qiáng)表達(dá)及bcl-2/bax比值的增加有關(guān)。

引用本文： 秦春宏,李永國(guó). 重復(fù)加熱對(duì)肝癌HepG2細(xì)胞增殖周期及bcl-2的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006, 13(3): 295-297下轉(zhuǎn)304. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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