• 山東大學(xué)齊魯醫(yī)院普外科（濟南250012）;

目的研究survivin反義寡核苷酸(ASODN)對膽囊癌細胞凋亡的誘導(dǎo)作用。
方法采用脂質(zhì)體介導(dǎo)survivin ASODN轉(zhuǎn)染人膽囊癌細胞株GBC-SD，采用流式細胞儀技術(shù)檢查細胞凋亡變化，采用電鏡技術(shù)觀察細胞的形態(tài)變化，采用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)（RT-PCR）檢測survivin基因表達的變化。
結(jié)果脂質(zhì)體介導(dǎo)轉(zhuǎn)染survivin ASODN后，膽囊癌細胞內(nèi)survivin mRNA表達相對系數(shù)為0.512±0.011,明顯低于未轉(zhuǎn)染者的0.980±0.012 (P＜0.01)，平均下調(diào)了47.83%(P＜0.01)，凋亡細胞比率為(26.28±3.91)%，明顯高于未轉(zhuǎn)染者的（0.50±0.23）%，P＜0.01，電鏡下可見膽囊癌細胞呈典型凋亡樣改變。
結(jié)論survivin ASODN轉(zhuǎn)染后能下調(diào)survivin的表達，可有效誘導(dǎo)GBC-SD細胞凋亡。

引用本文： 馮立民,姜希宏,烏新林,王建立,壽楠海. survivin反義寡核苷酸(ASODN)誘導(dǎo)膽囊癌細胞凋亡的研究. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(3): 298-301. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Adida C, Crotty PL, McGrath J, et al. Developmentally regulated expression of the novel cancer antiapoptosis gene survivin in human and mouse differentiation [Ｊ]. Am J Pathol, 1998; 152(1)∶ 43.
2. Todoroki T, Kawamoto T, Takahashi H, et al. Treatment of gallbladder cancer by radical resection [Ｊ]. Br J Surg, 1999; 86(5)∶622.
3. Hay BA, Wassarman DA, Rubin GM. Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death [Ｊ]. Cell, 1995; 83(7)∶1253.
4. ZangemeisterWittke U, Simon HU. An IAP in action: the multiple roles of survivin in differentiation, immunity and malignancy [Ｊ]. Cell Cycle, 2004; 3(9)∶1121.
5. Li F, Ambrosini G, Chu EY, et al. Control of apoptosis and mitotic spindle checkpoint by survivin [Ｊ]. Nature, 1998; 396(6711)∶580.
6. Sarela AI, Macadam RC, Farmery SM, et al. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma [Ｊ]. Gut, 2000; 46(5)∶645.
7. 王治, 梅柱中, 童新， 等. 靶向survivin的反義寡核苷酸對腫瘤細胞增殖的抑制作用 [Ｊ]. 中國生物化學(xué)與分子生物學(xué)報, 2001; 17(6)∶795.
8. 羅順峰, 朱虹, 張萬廣, 等. 5FU對肝癌細胞內(nèi)凋亡抑制蛋白survivin的誘導(dǎo)作用 [Ｊ]. 肝膽外科雜志, 2002; 10(3)∶227.
9. Ikeguchi M, Liu J, Kaibara N. Expression of survivin mRNA and protein in gastric cancer cell line (MKN45) during cisplatin treatment [Ｊ]. Apoptosis, 2002; 7(1)∶23.
  1. 1. Adida C, Crotty PL, McGrath J, et al. Developmentally regulated expression of the novel cancer antiapoptosis gene survivin in human and mouse differentiation [Ｊ]. Am J Pathol, 1998; 152(1)∶ 43.
  2. 2. Todoroki T, Kawamoto T, Takahashi H, et al. Treatment of gallbladder cancer by radical resection [Ｊ]. Br J Surg, 1999; 86(5)∶622.
  3. 3. Hay BA, Wassarman DA, Rubin GM. Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death [Ｊ]. Cell, 1995; 83(7)∶1253.
  4. 4. ZangemeisterWittke U, Simon HU. An IAP in action: the multiple roles of survivin in differentiation, immunity and malignancy [Ｊ]. Cell Cycle, 2004; 3(9)∶1121.
  5. 5. Li F, Ambrosini G, Chu EY, et al. Control of apoptosis and mitotic spindle checkpoint by survivin [Ｊ]. Nature, 1998; 396(6711)∶580.
  6. 6. Sarela AI, Macadam RC, Farmery SM, et al. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma [Ｊ]. Gut, 2000; 46(5)∶645.
  7. 7. 王治, 梅柱中, 童新， 等. 靶向survivin的反義寡核苷酸對腫瘤細胞增殖的抑制作用 [Ｊ]. 中國生物化學(xué)與分子生物學(xué)報, 2001; 17(6)∶795.
  8. 8. 羅順峰, 朱虹, 張萬廣, 等. 5FU對肝癌細胞內(nèi)凋亡抑制蛋白survivin的誘導(dǎo)作用 [Ｊ]. 肝膽外科雜志, 2002; 10(3)∶227.
  9. 9. Ikeguchi M, Liu J, Kaibara N. Expression of survivin mRNA and protein in gastric cancer cell line (MKN45) during cisplatin treatment [Ｊ]. Apoptosis, 2002; 7(1)∶23.